GAL and cyst: In the first stage or foundation stage, Jacob lectins bind to the plasma membrane Gal/GalNAc lectins; in the second or wattle stage, Jacob lectins cross-link the chitin fibrils that are deposited on the surface of encysting amoeba and finally, in the third or daub stage, the cyst wall is solidified by the addition of the Jessie-3 lectin resulting in an impermeable and rigid cell wall (Chatterjee et al., 2009).