2000), in the sera of NAFLD model rats. This finding suggests a new mechanism by which NAFLD lowers testosterone, LH and FSH levels. In addition, NAFLD exaggerated the oxidative stress response as evidenced by the lowered testicular levels of GSH and SOD activity, leading to significant alterations in the histopathology of the rat testes, an effect that has been described in various other studies on HFD-fed animals (Hammoud et al. 2008; Du Plessis et al. 2010; Alhashem et al. 2014). Here, PLOD1 is linked to metabolic dysfunction-associated steatotic liver disease.