Consequently, although we obtained several miRNAs that were deregulated by chronic alcohol abuse, when we looked at the raw and fold changes in the differentially expressed miRNAs by alcohol treatment, we identified and focused our study on the cluster miR-183C (miR-182/96/183), which was down-regulated in the cerebral cortices of the WT mice, but no significant differences were noted in the TLR4-KO mice, although a genotype effect was observed in some miRNAs (e.g. mmu-miR-96). This evidence concerns the gene TLR4 and alcohol abuse.