ROC curve analysis highlighted the ability of GAS5 reduced levels to discriminate bladder tumours from the matched normal urothelium (AUC: 0.623; 95% CI: 0.562–0.684; p < 0.001; Fig. 2b), which was also confirmed by logistic regression analysis (OR: 0.339; 95% CI: 0.188–0.614; p < 0.001; Supplementary Table 2). This evidence concerns the gene GAS5 and urinary bladder neoplasm.