Similarly, in our T cell-mediated melanoma cell killing assays, apigenin-treated A375 cells displayed enhanced sensitivity towards activated Jurkat T cells, with a correlated increase of secreted IL-2 detected from co-cultures, which indicated that apigenin-incurred PD-L1 downregulation in A375 cells could elicit similar responses as checkpoint blockade. The gene discussed is IL2; the disease is melanoma.