HDGFL2 and infection: Therefore, the seasonal fluctuations in RDT performance in the present study can be attributed to lack or least mixed infection of PfHRP2-negative isolates with RDT sensitive strain in low transmission period undermining the detection of P. falciparum infection by RDT, withholding anti-malarial treatment in RDT negative malaria patients leading to rise in transmission of PfHRP2-negative isolates and low parasitaemia level resulting in insufficient production of detectable HRP2 besides other factors.