HDGFL3 and coinfection: Although the reason for such seasonal fluctuation in frequency distribution of hrp2 and hrp3 deletion parasite strains is not known, it is expected that more of the detection failure by RDT in low transmission period of summer would be due to hrp2 deletion because of its high prevalence in the region compared to hrp3, reduced co-infection (either with RDT sensitive, hrp3 negative parasites or both) and successful treatment of patients infected with RDT sensitive parasites or HRP3 negative but HRP2 positive parasites (as HRP2 antigen is the main target for RDT detection) upon detection by RDT.