To determine the significance of Ly6G+CD11b+ cells for liver metastasis of pancreatic cancer, we used specific neutralizing antibodies to deplete Ly6G+ cells in two mouse models: MMC18-based model through injection of matrix gel-embedded cancer cells into spleen; Pan02 cells as another model [29,38,39]. The gene discussed is ITGAM; the disease is familial pancreatic carcinoma.