MMP9 and neoplasm: Additionally, matrix metalloproteinase 9 (MMP‐9) was significantly overexpressed in TBL20‐EV compared with BL20‐EV and shown to be a potential target of miR‐181a and b. MMP‐9 is upregulated at the “pre‐metastatic niche” in lung metastasis (Kaplan et al., 2005), and various MMPs have been shown to be present in tumour cell EV (Dolo et al., 1999; Hakulinen, Sankkila, Sugiyama, Lehti, & Keski‐Oja, 2008) and to have a role in metastasis of TBL20 cells (Adamson, Logan, Kinnaird, Langsley, & Hall, 2000).