To examine the mutation status of these cell lines, the following genes (AKT1, ATM, BARD1, BRCA1, BRCA2, BRIP1, CDH1, CHEK2, EPCAM, FAM175A, FANCC, MEN1, MRE11A, MLH1, MSH2, MSH6, MUTYH, NBN, NF1, ALB2, PIK3CA, PMS1, PMS2, PTEN, RAD50, RAD51C, RAD51D, STK11, TP53, and XRCCC2) were selected from the hereditary breast cancer panel of AmbryGenetics, GeneDx, and University of Washington. This evidence concerns the gene AKT1 and breast cancer.