When tumors start outgrowing existing vasculature, this result in glutamine deprivation and hypoxia within the tumor cells and ARD1 acetyl-transferase acetylates PGK1 at Lys388, which in turn phosphorylates Beclin1 at Ser30, leading to a conformational change and activation of class III phosphatidylinositol (PI) 3-kinase (VPS34) to produce phosphatidylinositol 3-phosphate (PI(3)P), facilitating the initiation of tumor-autophagy and promotes tumor development [27]. The gene discussed is PIK3C3; the disease is neoplasm.