In the next step, we examined the roles of IFN-γ and IL-6 in the pulmonary pathophysiology of DM RP-ILD by immunostaining and hematoxylin-eosin (H&E) staining of lung tissues, hilar lymph nodes, and spleen tissues from two patients from whom specimens were obtained on autopsy (Fig. 3). This evidence concerns the gene IFNG and retinitis pigmentosa 1.