RYR1 was mutated most frequently in both murine (4/5 = 80%) and human (5/37 = 13.5%) HCCs associated with NASH pathology, and RYR1 was less often mutated in other HCCs (7.37%, n = 353, TCGA data) (Fig. 7c). The gene discussed is RYR1; the disease is metabolic dysfunction-associated steatohepatitis.