Twenty-eight calcium signaling genes were mutated in NASH-HCCs, with 6 recurrently mutated (Ryr1, Ryr2, Cacna1d, Cacna1h, P2rx1 and Itpr1) all encoding calcium channel proteins, and Ryr1 was mutated in 4/5 samples (Fig. 6b). This evidence concerns the gene P2RX1 and metabolic dysfunction-associated steatohepatitis.