RYR1 and metabolic dysfunction-associated steatohepatitis: Eight genes showed significantly higher mutation frequencies in NASH-HCCs (the combined cohort (n = 37) or TCGA cohort (n = 20)) as compared to other HCCs not complicating NASH/NAFLD (TCGA, n = 353), and two of them encode calcium channel proteins (RYR1 and CACNA1H) (Fig. 7c).