To determine whether the changes in gene expression were primary or secondary responses after long-term of treatment with the HF diet or pioglitazone, we analyzed the expression of SREBP-1c, SCD-1, CD36, PPARγ2, and PCG1α at 6 h after pioglitazone treatment combined with vehicle or lipid injection (Figure 5). This evidence concerns the gene PPARG and hydrops fetalis.