SMAD4 and cancer: Furthermore, combine the analysis of somatic copy number alterations (SCNAs) and single nucleotide variants (SNVs) to infer Cancer Fractions (CCF) between tumor organoids and biopsies, revealing that CRC driver mutations were maintained in organoids and most commonly altered genes and were all represented in organoids, including APC, TP53, KRAS, PIK3CA, FBXW7, and SMAD4.