Finally, by using colony assay, we observed that, in accordance with Annexin V/PI and Trypan Blue results, the colony number does not significantly decrease in combined treatment compared to 5-Fluorouracil alone (Figure 3(c)) while the co-treatment with SLC-0111 significantly affects cancer cell proliferation as shown by the decreased diameter of the colonies upon CAIX inhibitor treatment (Figure 3(d)). The gene discussed is CA9; the disease is cancer.