Inflammasomes are cytoplasmic multiprotein complexes, including four individual inflammasome branches (NLRP1, NLRP3, NALP4 and AIM2).9, 10 Inflammasomes can be generated and activated by various stimulators,11 and E. coli and S. aureus identified in bovine mastitis are considered as main stimuli.12, 13 A few studies have reported that the complex network of NF‐κB and NLRP3 inflammasome played an important role in mastitis,14 but the specific mechanism between NF‐κB and NLRP3 inflammasome needs to be further identified. Here, NLRP4 is linked to mastitis.