In this study, the TALEN technology was applied to knock out the rat Eogt gene, which encodes the enzyme that catalyzes protein glycosylation, and demonstrated that this glycosylation deficiency could prevent Treg differentiation by suppressing Notch signaling, leading to CD4+ T lymphocyte infiltration that resulted in the aggravation of hepatic dysfunction in Con A-induced AIH. Here, CD4 is linked to autoimmune hepatitis.