Individual CpG site analysis showed that CASZ1_ CpG_2.3, CASZ1_CpG_4, CASZ1_CpG_5, CDH13_CpG_8, ING2_CpG_1.2.3.4, ING2_CpG_5 and ING2_CpG_6.7 were significantly hypermethylated in ESCC tissues as compared with controls (Table 5). The gene discussed is CASZ1; the disease is esophageal squamous cell carcinoma.