Since PTK7 acts as a regulatory factor of the canonical Wnt/β-Catenin pathway, we analyzed the correlation between PTK7 and β-Catenin expression by immunohistochemistry (IHC), whose results showed that normal tissues and HNSCC tissues displayed concordant negative, weak, moderate, and strong PTK7 and β-Catenin staining (Fig. 1d). This evidence concerns the gene PTK7 and head and neck squamous cell carcinoma.