MAPK8 and esophageal squamous cell carcinoma: To test the effect of RIT1 on these two pathways, WB was used to study the expression levels of phosphorylated extracellular signal–regulated kinase (ERK), c-Jun NH2-terminal kinase (c-JNK), P38, and AKT after exogenous knockdown and overexpression of RIT1 in ESCC cells.