Conversely, anti-miR inhibition of miR-218 in CM-producing MDA-231-bone cells led to reduction of SMAD2/3 phosphorylation in cancer cells (Fig. 4a) and de-repression of SMAD2/3 signaling in preosteoblasts (Fig. 4b) at the 2-h time point. This evidence concerns the gene SMAD2 and cancer.