Indeed, there was a ~ 5-fold and 2.5-fold increase in Iba1+ cells and GFAP+ staining in CIT + CON mice at 18 weeks compared to the 9-week cohort (Fig. 5h, i), suggesting ageing supersedes high-fat or hyperglycemia-induced inflammation on the brain. This evidence concerns the gene GFAP and Hyperglycemia.