INS and diabetes mellitus: This report examines the age dependence of acute STZ toxicity and describes the long-term natural course of the model including glucose, HbA1C, lipid, metabolic hormones (such as insulin, glucagon), cytokine levels and clinical conditions, mortality, natural recovery, and multiple diabetic complications resulting from the end-stage of uncontrolled diabetes including the description of novel, stress-free, reproducible behavioral endpoints.