MSP analysis was performed on all the 96 WTs for the promoter region of HACE1 and CDKN2A and CDKN2B. MSP of the CpG-177 island of HACE1, previously reported to be frequently methylated in WTs [12, 13], revealed the presence of PCR products from both methylated and unmethylated DNA in 81 tumours (84%), and of the product from unmethylated DNA only in the remaining 14 samples. This evidence concerns the gene CDKN2A and neoplasm.