Furthermore, our finding also demonstrated that epigenetic regulations, including DNA methylation of HSPB1, ALDOB, and RPL30, led to changes in the DNA methylation profiles of HSPB1 (p value ≤ 1.00 × 10−3), ALDOB (p value ≤ 0.85), and RPL30 (p value ≤ 1.00 × 10−3), which resulted in significant changes in the gene expression profiles of HSPB1 (p value ≤ 1.00 × 10−3), ALDOB (p value ≤ 1.5 × 10−2), and RPL30 (p value ≤ 1.00 × 10−3) in the progression from NAFLD&NASH to HCC, as shown in Figure 6. The gene discussed is HSPB1; the disease is metabolic dysfunction-associated steatotic liver disease.