In line with that, other breast cancer assays, like the GeneXpert Breast Cancer STRAT4 assay from Cepheid, that also measures the expression levels of ERBB2, ESR1, PGR and MKI67, found that macrodissection of whole tissue sections is not required for accurate assessment of these genes by RT-qPCR [32]. This evidence concerns the gene ESR1 and breast cancer.