The study by Castellsague et al. including 1042 laryngeal cancer patients from 29 countries tested the specimens with PCR and a DEIA for the presence of HPV-DNA and samples containing HPV-DNA were further subject to HPV E6*I mRNA detection and to p16INK4a, pRb, p53, and Cyclin D1 immunohistochemistry [25]. The gene discussed is CCND1; the disease is laryngeal carcinoma.