A few recent reports showed that gemcitabine treatment fortified the stemness of cancer cells through the Nox/ROS/NF‐κB/STAT3 signalling cascade and the lncRNA HOTAIR.19, 42 Similarly, our data indicate that gemcitabine treatment promotes the sphere formation ability and up‐regulates CSC marker expression in the PC cell lines MiaPaCa‐2 and Panc‐1. The gene discussed is NFKB1; the disease is pachyonychia congenita.