In a multivariable logistic regression analysis the change in TGF-β1 remained significant, OR 0.85 (95% CI 0.75–0.96) when age, hypertension and mean heart dose were included in the model to test variables associated with ≥15% and < 15% decline in TAPSE (Additional file 5: Table S5). The gene discussed is TGFB1; the disease is hypertensive disorder.