Here, we provided for the first time clear evidence that α-Hed induces autophagosome accumulation by altering the lysosomal pH and inhibiting lysosomal cathepsin maturation, as evidenced by the following: (i) α-Hed treatment resulted in an increased number of autophagosomes in human NSCLC cells (Figure 1); (ii) α-Hed inhibited human NSCLC cell autophagic flux (Figure 2); (iii) α-Hed altered the lysosomal pH markedly, attenuating the enzymatic maturation of lysosomal cathepsins (Figure 3). The gene discussed is NKAPD1; the disease is non-small cell lung carcinoma.