To verify the inhibitory effect of α-Hed on autophagic flux in NSCLC cells, we transfected the cells with a tandem reporter plasmid expressing mCherry-GFP-LC3 fusion protein and treated the cells with α-Hed (12.5 μM), Baf (0.1 μM; autophagic flux inhibitor; 24 h), or HBSS (autophagic flux inducer; 6 h). Here, NKAPD1 is linked to non-small cell lung carcinoma.