Dysregulated expression of Pknox1 was shown to be associated with glucose and lipid dysmetabolism status of T2DM complicated with NAFLD.9, 19 In the rat model of STZ and HFD‐induced T2DM/NAFLD, we also found that the hepatic level of Pknox1 protein was significantly upregulated in consistent with the progression of hepatic steatosis and IR. This evidence concerns the gene PKNOX1 and fatty liver disease.