Given that the combination of NU7441 and M1 elicit ER stress-induced ICD, we sought to analyze the composition and activation status of tumour microenvironment after the combined therapy in two immunocompetent mouse models with MC38 colon cancer and Pan02 pancreatic ductal adenocarcinoma cells (our data demonstrated that DNA-PK inhibitor can improve the oncolytic efficacy of M1 virus in both two mouse tumour cell lines in vitro, shown in Supplementary Fig. 5). The gene discussed is PRKDC; the disease is pancreatic ductal adenocarcinoma.