SGK1 and breast cancer: Next, we validated SGK1 as a target of miR-29a and miR-101-1 by co-expressing the microRNAs along with firefly luciferase reporter genes cloned upstream to 3′-UTR of SGK1. Ectopic expression of both of the microRNAs decreased the firefly luciferase activity in 293FT cells expressing the 3′-UTR of SGK1. Consistent with the findings, transfection with anti-miRs targeting miR-29a and miR-101-1 not only rescued the repression of luciferase activity in 293FT cells (Fig. 2A) but also led to sustained expression levels of SGK1 based on Western blot analysis of breast cancer cells (Fig. 2B).