Tilapia GH gene expression was confirmed by reverse transcriptase PCR in the first-generation (F1) three days post-fertilization (3 dpf) larvae, and F1 one-year-old acromegaly muscle and liver (Table S1 and Figure 2A,B), but not in WT larvae or one-year-old WT fish. Here, GH1 is linked to acromegaly.