In order to assess the effect of sera of CKD patients on ROS, NO, and TNF-α release, in C6 cells, we treated the cells with sera from eighteen different subjects: four healthy people (H1, IS = 2.32 μM; H2, IS = 2.91 μM; H3, IS = 4,52 μM; H4, IS = 5.38 μM), eight CKD patients (CKD1, IS = 7.48 μM; CKD2, IS = 11.70 μM; CKD3, IS = 28.44 μM; CKD4, IS = 31.46 μM; CKD5, IS = 33.67 μM; CKD6, IS = 38.13 μM; CKD7, IS = 38.73 μM; CKD8, IS = 39.55 μM) and six CKD dialysed patients (HD1, IS = 46.50 μM; HD2, IS = 50.44 μM; HD3, IS = 53.23 μM; HD4, IS = 54.25 μM; HD5, IS = 62.08 μM; HD6, IS = 70.74 μM). The gene discussed is TNF; the disease is chronic kidney disease.