When FISH results were analysed jointly with IHC (Fig 2C), rate of overexpression/amplification of HER3 in membrane and cytoplasm increased up to 16% [AMP (1 patient), GBC (1 patient), ICC (6 patients), ECC (3 patients)] and 24% [AMP (1 patient), GBC (1 patient), ICC (10 patients), ECC (4 patients)], respectively. Here, ERBB3 is linked to intrahepatic cholangiocarcinoma.