Consistent with the result of TWAS, previous studies have reported 4 of the 19 genes (PPP2R2A, E2F3, KCNK5, and SOX4) play important roles in the development of PC,20, 21, 22, 23 and 2 of the 19 genes (CHSY1 and EGLN3) have been proved associated with PC via bioinformatics methods.25, 26 SOX4 is significantly associated with PC and overexpressed in PC patients. This evidence concerns the gene E2F3 and pachyonychia congenita.