To confirm whether miR-6775-3p inhibits ESCC cell proliferation, migration, and invasion through targeting MAGE-A family, TE1 cells were co-transfected with miR-6775-3p mimics plus MAGE-A1 over-expression plasmids, and cell migration and invasion were evaluated. This evidence concerns the gene MAGEA1 and esophageal squamous cell carcinoma.