These results were confirmed by qRT-PCR analysis, showing upregulated expression of IFNB, MX1, IP-10, CH25H, ISG15, OASL, IFITM3, RIG-I, and TRIM21 following ZIKV infection at 24 hpi but not 12 hpi (Fig. 4e and Fig. S11b–g), in agreement with previous findings in A549 cells29. This evidence concerns the gene IFNB1 and Zika virus infectious disease.