Nevertheless, independent of SHM evident at day 7 p.i., these ASC do not appear to be migration competent, as tdTomato+ ASC do not emerge in the CNS until day 14 p.i. Irrespective of migration capacity, these results are consistent with preferential retention of Ag-induced, long-lived IgM ASC in the spleen following peripheral immunization or infection (12). This evidence concerns the gene CD40LG and infection.