AIF1 and neoplasm: They are normal tissue (N), where microglia were of low density and in a typical ramified shape with small soma; tumor rim (TR), where microglia were of increased density with thicker branches and enlarged soma; invasive margin (IM), where a large number of microglia gathered at the leading edge of tumor invasion; and intratumoral region (IT), where Iba1+ myeloid cells were typically amebiform (Fig. 2a).