More recently, an rAAV8 D377Y-mPCSK9 (PCSK9DY) mouse model (on a male C57BL/6 background) has been used to study atherosclerosis [93–95], where a single injection of AAV-mPCSK9DY is sufficient to cause long-term atherosclerosis in mice comparable to LDLR−/− strains, without the need to create a transgenic mutant knock-in line [93]. The gene discussed is LDLR; the disease is atherosclerosis.