As for the clinicopathological features, although the statuses of hormone receptors (ER and PR) and PAM50 subtype were associated with DNAm age, they only had a minor effect on the associations between DNAm age and breast cancer prognosis, indicating that the effect of DNAm age on breast cancer prognosis was not likely to mediate through the clinicopathological features. This evidence concerns the gene NR4A1 and breast cancer.