To create cells with and without high concentrations of defined supervillin isoforms, we transfected U2OS osteosarcoma or RH30 rhabdomyosarcoma cells with plasmids encoding EGFP-hSV1, EGFP- or Flag-tagged hSV4, or GFP-mSV2 (murine supervillin, isoform 2; archvillin; MAV) [14, 31]. The gene discussed is SVIL; the disease is osteosarcoma.