The multitarget tyrosine kinase inhibitor GZD824 exhibited an antitumor effect in pre-B-ALL by inhibiting both the SRC kinase and PI3K/AKT/mTOR pathways, and ALL cells with lower IRS1 expression were more sensitive to GZD824 treatment than those with higher IRS1 expression. The gene discussed is MTOR; the disease is acute lymphoblastic leukemia.