TNF and rheumatoid arthritis: For this purpose, peripheral blood samples were obtained from healthy controls (HC, n = 25), untreated RA patients (untreated, n = 26) and RA patients treated with methotrexate (MTX, n = 20), TNF inhibitors (anti-TNF, n = 20), and the JAK inhibitor Tofacitinib (TOFA, n = 14).