GPC3 and hepatocellular carcinoma: Furthermore, as shown in Figure 1C, it was found that GPC3-CAR T cells showed the significantly (P < 0.001) stronger cytotoxicity on HCC cell PLC/PRF/5 compared with the UTD, and the cytotoxicity was enhanced with the increase of effector:target ratio from 1:3 to 3:1, indicating that the cytotoxicity of GPC3-CAR T cells was dose-dependent.