GPC3 and neoplasm: In order to investigate the effect of PD-1 deficient GPC3-CAR T cells on the tumor-related genes expression in xenograft tumors established with PLC/PRF/5, quantitative reverse transcription PCR was carried out to characterize the mRNA expression levels of tumor-related genes of CCND1 (cyclin D1), CTNNB1 (catenin beta-1) and MET (MET proto-oncogene, receptor tyrosine kinase) in xenografts treated with various genetically engineered (or not) T cells or PBS.