The ratio of phospho-Akt/Akt in the PD-1 deficient GPC3-CAR T cells was 4.35 times higher than that in the wild-type GPC3-CAR T cells, and meanwhile, the expression level of Bcl-xL in the PD-1 deficient GPC3-CAR T cells was 1.86 times higher than that in the wild-type GPC3-CAR T cells after 48 h of coculture with native PD-L1-expressing GPC3-positive PLC/PRF/5 HCC cells. The gene discussed is CD274; the disease is hepatocellular carcinoma.