By determining the optimal cut-off using ROC curves for each molecule, the individual sensitivity and specificity of these six molecules (NANOG, CD49f, LGR5, ΔNp63, SOX2, and CD24) for cancer detection ranged from 29.2% to 62.5% and 83.3 to 100%, respectively (Supplementary Table S2). This evidence concerns the gene NANOG and cancer.