Taken together, our findings indicate that miRNA-302a/d can inhibit the stemness and proliferation of HCC cells through targeting E2F7 and downstream AKT/β-catenin/CCND1 signaling and may play an essential role in HCC progression suggesting that miRNA-302a/d and E2F7 may be promising therapeutic targets for HCC patients. This evidence concerns the gene E2F7 and hepatocellular carcinoma.