The results indicated that high HSF1 expression in GC tissues was significantly correlated with larger tumour size (P = 0.001), advanced Bornmann classification (P = 0.002), advanced depth of invasion (P = 0.015), lymph node metastasis (P<0.001), distant metastasis (P = 0.011) and tumour-node-metastasis (P<0.001). This evidence concerns the gene HSF1 and gastric cancer.